manbextapp下载:TaPUB15, a U-Box E3 ubiquitin ligase gene from wheat, enhances salt tolerance in rice
Qiaoru Li, Bo Li, Jingyi Wang, Xiaoping Chang, Xinguo Mao, Ruilian Jing
Food and Energy Security, 31 October 2020, IF：5.242
Plant U-box (PUB) E3 ubiquitin ligases play essential roles in response to environmental stresses and hormone signaling, but little is known about them in wheat ( Triticum aestivum L.). We characterized a U-box E3 ubiquitin ligase gene, TaPUB15 , which is conserved in wheat cultivars. TaPUB15 was expressed in multiple tissues, and the expression was much higher in roots than in other tissues. Transcription of TaPUB15 was induced by salt, abscisic acid, 4°C, and polyethylene glycol (PEG). Overexpression of TaPUB15-D led to improve salt tolerance in transgenic rice, along with more roots in transgenic rice. Ion flux assays showed that transgenic rice plants had improved salt tolerance and maintained low Na+/K+ ratios under salinity stress. Gene expression assays indicated that several salt stress-related genes were significantly upregulated in transgenic rice relative to the wild type. These results from transgenic rice were also verified in transgenic Arabidopsis. Our data showed that TaPUB15 plays crucial roles in enhancing salt tolerance in crop plants and served a valuable gene resource for promoting the breeding of salt-tolerant wheat in the future.